4 research outputs found
Analysis og gene expression in prokaryotic and eukaryotic model organisms by proteomic gel-based separation tools
This PhD thesis showed the applicability of a gel-based proteomic separation tool, 2-D electrophoresis in three independent projects. Supplemented with results obtained using different techniques the proteomic studies enabled a global imaging of proteoms in the studied biological systems. Comparing total proteoms of E. coli 61 protein changes were identified and connected with the development of the bacterial population in the presence of an antibiotic compound, erythromycin. This classic proteomic approach included sample extraction, optimization of its 2D separation followed by 2D gel analysis and protein identification by MS methods. A disadvantage of this work was an enourmously large amount of data to be analyzed by computer analysis. For the study of membrane proteom of B. subtilis during a pH induced stress, on the other hand, a modification of isolation techniques for membrane and membrane associated proteins was required first to improve the subsequent protein separation by 2-D electrophoresis. The optimalization of protein extraction included changes in detergents used for protein solubilization and a prolongation of time periods in the protein solubilization protocol. 5 relevant protein changes were then described that play a role in the bacterial response to pH stress. The proteins were..
Counts and crude rates per 100,000 of accidental poisoning deaths among persons age 15 to 64, by selected characteristics.
<p>Counts and crude rates per 100,000 of accidental poisoning deaths among persons age 15 to 64, by selected characteristics.</p
PA County with Highest 2010–2014 Accidental Poisoning Mortality Rate by Race, Sex, and Age Group.
<p>PA County with Highest 2010–2014 Accidental Poisoning Mortality Rate by Race, Sex, and Age Group.</p
PA Age-Specific Accidental Poisoning Mortality Rates Per 100,000 by Race and Sex, 2010–2014.
<p>PA Age-Specific Accidental Poisoning Mortality Rates Per 100,000 by Race and Sex, 2010–2014.</p